This is the typical vaccination schedule and how to administer vaccinations used for pullet laying hens to prevent Marek's disease, Newcastle-Bronchitis, Infectious Bursal Disease, Layngotracheitis, Fowl Pox recommendations.

Pullet Laying Hen-Age in Days
Age: One day-Vaccination-Marek's Disease
Vaccination Location: Injection in subcutaneous back of neck

Age: 9-14 days-Newcastle-Bronchitis
Drinking water, eye drop or spray

Age: 14 days-Infectious Bursal Disease
Drinking water or Spray

Age: 28 days-Infectious Bursal Disease
Drinking water or Spray

Pullet Laying Hen-Age in Weeks
Age 4 weeks-Newcastle-Bronchitis
Drinking water or Spray

8 weeks-Laryngotracheitis
Fowl Pox
AE Eye drop
Wing web stab
Wing web stab

13-14 Weeks-Newcastle Bronchitis
Drinking water or spray

16 Weeks-Laryngotracheitis
Spray or eye drop

(Post Housing)Newcastle Bronchitis (or )Every 3 months in some operations
Spray or drinking water
16 Weeks-Inactivated Newcastle-Bronchitis
​Injected IM/SQ (Jurikson, 1999).

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More About Vaccine's For Chickens

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References: Jurikson, Matthew (Student). Dr. Eckroade, Dr. Davison, Dr. Gingerich (Faculty). (1999) University of Pennsylvania School of Veterinary Medicine. Retrieved on Oct. 7, 2016. from Typical vaccination schedule for laying hens. http://cal.vet.upenn.edu/projects/poultry/Syllabus/page16.htm