Step by Step Instructions and video on how to administer a wing web puncture for chickens and turkeys. We do recommend that you have a licenced livestock or poultry vet administer vaccine's, however if you are in need of the proper instructions for administering a wing web puncture for chickens or turkeys the following is the correct method.

How to Administer A Wing-web Puncture for Fowl Pox (pigeon pox), and Avian Encephalomyelitis, Fowl Cholera chickens.
​Use double-prong sewing machine needle (supplied by vaccine manufacturer)
Dip into vaccine before each stab, spread the wing to expose the underside (up), stab through, do not touch feather with needles, avoid vessels.
For fowl pox, examine for "take" 6-10 days post, swelling followed by scab formation.
Re-vaccinate non-reactor flock. ​
​Vaccinating Turkeys:To vaccinate turkeys, puncture the loose skin between the thigh and abdomen.

Featured Video: How to do Fowl Pox Vaccine

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More About Vaccines For Chickens

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References: 
1. Video: Guate, Ray09. (May 15, 2015). How to do fowl pox vaccine. https://www.youtube.com/watch?v=UXt34m12CrM
2. Jurikson, Matthew (Student). Dr. Eckroade, Dr. Davison, Dr. Gingerich (Faculty). (1999) University of Pennsylvania School of Veterinary Medicine. Retrieved on Oct. 7, 2016. Methods of poultry vaccination individual bird application. ​http://cal.vet.upenn.edu/projects/poultry/Syllabus/page11.html